Thursday, 11 October 2018

Selecting the right microscope

BA Elite Series


Category: Advanced Transmitted Light Microscopes

Target specimen: Transparent samples (sections, smears, emulsions, water samples) from Biology & Medicine; rarely from Industry

Target customer: Lab technicians

This comparison is an approach for classifying different microscope models in a defined performance range. Here we are talking about Motic’s BA Elite series of Advanced Transmitted light microscopes, meant for routine work in university and biomedical lab. The comparison is meant to help you in finding a suitable solution for your lab.

The following chart is based on the specifications of the basic outfit for each model and its upgrade options. Our rating of the single specifications rests upon an individual understanding of the actual numerical values. This is a subjective rating, and we understand that each person might have its own opinion.


BA SERIES - Comparison Chart


All technical features are judged on the impact in practical microscopical work. Two examples: A system with Field of View 22 (BA410E) presents 21% more visible area than a FOV 20 (BA210E, BA310E). A quicker overview, less work to move the X/Y stage, faster results: we rated this feature with a score of “****”. The stage size, although different in BA210E vs. BA310E/BA410E, has been rated with an equal score (***). Does the stage size really affect the efficiency of daily microscopical work? The travel range seems to be more important and thus has been classified with individual scores.

Upgradeability is also ranked following the practical impact in laboratory work. A turret condenser gives more flexibility, but by performance is not superior to a slider solution.

The final chart displays an evident fact: The models BA210E and BA310E are close to each other in performance, significantly separated only by the Koehler illumination. BA410E is far beyond, especially regarding upgradeability, although some features (no swiveling eyepiece tube, no Halogen/LED interchangeability in 100W version) deserve a low rating.


Some remarks:


Interpupillary distance
Young people and female users by nature have a small eye distance. For a long-term work, the tube should be adjusted properly. BA310E offers the broadest range in interpupillary distance. A microscope user should know his own value before purchasing a microscope.

Field of View
The larger, the better. The calculation is as follows: Visible Area (mm2) = ¶ x r2. An advantage of 21% can be granted to BA410E in comparison to BA210E/BA310E. 21% more visible area at one view without moving the X/Y-stage: a time saving feature, especially interesting in case of a high sample throughput or a fast review of large samples.
For more information about the FOV click here.

Trinocular beam split
A 100:0/0:100 split with a 100% light option to the camera port is recommended in case of light consuming contrast methods, especially for Fluorescence. The BA410E with its triple beam split option offers maximum flexibility for all observation/documentation cases.

Objectives
The necessary quality of objectives is deeply depending on the application. No description can beat a test with own samples. As objectives are a major price point, the user may think about a mixture of objectives, choosing a first-class objective (in most cases probably a 40X) as a workhorse and a set of additional objectives.

Objective range
The EC-H 2X objective in BA410E offers a visible area of 11mm diameter, the size of a thumbnail. Perfectly matching requests from pathology, but also able to close the gap between compound and stereo microscope magnification range in terms of visible field.

Fine focus
The minimum increment of the fine focus is essential for the evaluation of the sample in Z direction. The smaller the value, the better. With a small increment, it is much easier to get a 3D impression even from sections when focusing.

Illumination
The more power, the better the chances for the old fashioned Multi-Viewing Devices. Almost extinct, but still the best setup for an individual teaching. This hardware is the only way to transfer the complete Field of View to a second/third person. Any camera solution will lose plenty of visible field.

Halogen/LED interchangeability
LED illumination is the modern approach in terms of life-time and color temperature. For image documentation, modern digital cameras have got several tools to compensate the disadvantage of Halogen illumination in terms of color temperature: White balance.

Koehler illumination
A difficult case. Even on university level, some teachers do not know the importance of a proper Koehler setup for the evaluation of tiny, thin and hardly stained structures. This hardware addition (e.g. Field diaphragm) is going to become neglected. A clear recommendation from our side: If possible, the customer should go for a microscope with a Full Koehler illumination.
For more information about the Koehler Illuminatioon click here.

Fluorescence
HBO Fluorescence is still more flexible than most LED solutions. New dyes, multiple staining: the most variable setup can be achieved with a Mercury light source. For standardized Fluorescence applications with a single staining, LED Fluorescence may be adequate. Especially in educational environments, LED is strongly privileged. No need to care for the Mercury disposal of old bulbs or the risk of breaking a bulb.


Summary:


The selection of a specific model always depends on impartial demands and individual preferences. Depending on each individual needs and the application range, there is more than one correct decision to solve a specific application case.

_______________________________________________________


Comparison | BA Elite Series


BA210E


Model BA210E Rate
Optical system CCIS® ***
Observation system Swiveling Siedentopf;
30° inclination; 360° rotating
***
Interpupillary distance (mm) 55-75 ***
Eyepieces (Field of View) N-WF 10X/20 ***
Diopter adjustment +/- 5 dpt ***
Trino light split 100:0/20:80 ***
Nosepiece Reversed quadruple nosepiece **
Standard objectives EC Plan Achromats ***
Objective magnification range 4X up to 100X ***
Optional objectives No **
Positive and negative phase objectives EC-H Plan Phase ***
Objective mounting thread RMS (W 4/5'' X 1/36'') Yes **
Built-in coaxial mechanical stage with sample holder Yes **
Stage size (mm) 150 x 150 ***
Mechanical stage x&y range (mm) 80 x 30 **
Upper limit stop Preset; adjustable ***
Condenser (Focusable Abbe type) N.A. 1.25 + iris diaphragm + slot for contrast sliders **
Focus mechanism Coaxial; tension adjustment ***
Minimum fine focus precision (µm) 2 ***
Z-axis movement (mm) 20 ***
Filter holder with fixing cap Yes ***
Illumination 30W Halogen; 3W LED ***
Halogen / LED interchangeability Yes ***
Illumination position Built-in ***
Koehler illumination No **
Multi-Viewing devices No **
Transformer Built-in ***
Power supply 110-240V (CE) ***
Auto OFF No **
Light memory No **
Filters included Blue **
Dimensions (mm) 360 x 220 x 395 **
Weight (Kg) 7,2 **
Contrast techniques
Bright Field Yes **
Phase Contrast Slider ***
Simple Dark Field Slider ***
Simple POL Contrast Yes ***
Fluorescence HBO No **
Fluorescence LED Yes ***



BA310E


Model BA310E Rate
Optical system CCIS® ***
Observation system Swiveling Siedentopf;
30° inclination; 360° rotating
***
Interpupillary distance (mm) 48-75 ****
Eyepieces (Field of View) N-WF 10X/20 ***
Diopter adjustment +/- 5 dpt ***
Trino light split 100:0/20:80; optional 100:0/0:100 ****
Nosepiece Reversed quintuple nosepiece ***
Standard objectives EC Plan Achromats ***
Objective magnification range 4X up to 100X ***
Optional objectives EC Plan Achromat NCG ***
Positive and negative phase objectives EC-H Plan Phase ***
Objective mounting thread RMS (W 4/5'' X 1/36'') Yes **
Built-in coaxial mechanical stage with sample holder Yes **
Stage size (mm) 180 x 170 ***
Mechanical stage x&y range (mm) 80 x 55 ***
Upper limit stop Preset; adjustable ***
Condenser (Focusable Abbe type) N.A. 0.90/1.25 + iris diaphragm + slot for contrast sliders ***
Focus mechanism Coaxial; tension adjustment ***
Minimum fine focus precision (µm) 2 ***
Z-axis movement (mm) 20 ***
Filter holder with fixing cap Yes ***
Illumination 30W Halogen; 3W LED ***
Halogen / LED interchangeability Yes ***
Illumination position Built-in ***
Koehler illumination Yes ***
Multi-Viewing devices Dual/3-head/5-head ****
Transformer Built-in ***
Power supply 110-240V (CE) ***
Auto OFF No **
Light memory No **
Filters included Blue **
Dimensions (mm) 400 x 220 x 400 **
Weight (Kg) 8,6 **
Contrast techniques
Bright Field Yes **
Phase Contrast Slider & Turret ***
Simple Dark Field Slider & Turret ***
Simple POL Contrast Yes ***
Fluorescence HBO No **
Fluorescence LED Yes ***


BA410E


Model BA410E Rate
Optical system CCIS® ***
Observation system Siedentopf; 30° inclination; 360° rotating **
Interpupillary distance (mm) 50-75 ***
Eyepieces (Field of View) N-WF10X/22 ****
Diopter adjustment +/- 5 dpt ***
Trino light split 100:0/20:80; optional 100:0/0:100 or 100:0/20:80/0:100 *****
Nosepiece Reversed sextuple nosepiece ****
Standard objectives EC-H Plan Achromats ****
Objective magnification range 2X up to 100X ****
Optional objectives Plan FLUOR, Plan APOCHROMAT objectives *****
Positive and negative phase objectives EC-H Plan Phase ***
Objective mounting thread RMS (W 4/5'' X 1/36'') Yes **
Built-in coaxial mechanical stage with sample holder No; separate rackless stage ***
Stage size (mm) 180 x 170 ***
Mechanical stage x&y range (mm) 80 x 55 ***
Upper limit stop Preset; adjustable ***
Condenser (Focusable Abbe type) Swing-out Abbe N.A. 0.90/0.13 with iris diaphragm ***
Focus mechanism Coaxial; tension adjustment ***
Minimum fine focus precision (µm) 1 *****
Z-axis movement (mm) 27 ****
Filter holder with fixing cap Yes ***
Illumination 50W Halogen / 100W Halogen ****
Halogen / LED interchangeability Only 50W version ***
Illumination position External ***
Koehler illumination Yes ****
Multi-Viewing devices Dual/3-head/5-head ****
Transformer External in 100W ***
Power supply 110-240V (CE) ***
Auto OFF Yes ***
Light memory Yes ****
Filters included Blue **
Dimensions (mm) 550 x 242 x 435 (50W); 592 x 242 x 435 (100W) **
Weight (Kg) 14,2 **
Contrast techniques
Bright Field Yes **
Phase Contrast Slider & Turret ***
Simple Dark Field Slider & Turret ***
Simple POL Contrast Yes ***
Fluorescence HBO Yes ****
Fluorescence LED Yes ***

No comments:

Post a Comment